Isolation of a GABA receptor from bovine brain using a benzodiazepine affinity column

FEBS Letters

Sigel, Erwin¹  Eric A, Barnard¹  Mamalaki, Cleanthi¹ 

[1] Department of Biochemistry, Imperial College, London, SW7 2AZ, England

关键词: GABA receptor;    Benzodiazepine receptor;    Isolation;    Affinity chromatography;    GABA;    γ-aminobutyric acid;    BZ;    benzodiazepine;    EDTA;    ethylene-diamine-N;    N;    N';    N'-tetraacetic acid;    HEPES;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    SDS—PAGE;    sodium dodecylsulphate—polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(82)81008-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Benzodiazepine receptor solubilized from bovine cortical membranes was bound to a new benzodiazepine affinity column, the synthesis of which is described. Bio-specific elution with the benzodiazepine compound chlorazepate resulted in the elution of fractions highly enriched in specific binding for the GABA receptor agonist muscimol. Specific activity for [³H]muscimol binding was >1.3 nmol/mg protein. It is shown that [³H]flunitrazepam binding activity can be recovered by removal of chlorazepate from the purified fraction. These results strongly support a model which suggests that the 2 binding sites reside on the same physical entity.

【 授权许可】

Unknown