Use of a rapid DNA sequencing system to demonstrate the induction of frameshift mutations by bleomycin

FEBS Letters

Demopoulos, N.³  Scazzocchio, C.¹  Wayne Davies, R.² 

[1] University of Essex, Department of Biology, Colchester CO4 3SQ, England;University of Manchester Institute of Science and Technology, Applied Molecular Biology Group, Department of Biochemistry, PO Box 88, Manchester M60 1QD U.K.;University of Patras, Laboratory of Genetics, Patras, Greece

关键词: DNA sequencing M13;    Mutagenesis screen;    Bleomycin;    Frameshift;    Carcinogenesis;   

DOI  :  10.1016/0014-5793(82)80956-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rapid DNA sequencing system based on the single-stranded bacteriophage M13 and the chain-terminator method has been used to look directly for mutational alterations. A small DNA fragment that primes DNA synthesis through the N-terminal 200 base pairs of the β-galactosidase gene was prepared, and used to detect changes in base sequence among phages that give white plaques after treatment of the host cells with bleomycin. Bleomycin treatment of E. coli in which M13 mp2 was growing gave an increase in white plaque frequency. DNA sequence analysis of phage from 7 independent mutant plaques showed them all to have a frameshift mutation.

【 授权许可】

Unknown