The role of methylation in the taxis of Halobacterium halobium to light and chemo‐effectors

FEBS Letters

Bibikov, S.I.¹  Glagolev, A.N.¹  Baryshev, V.A.¹ 

[1] Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR

关键词: Halobacterium halobium photobehavior;    ΔH +-sensing;    Protein methylation;    Blue-light taxis;    CCCP;    m-chlorocarbonylcyanidephenylhydrasone;    MCP;    methyl-accepting chemotaxis protein;    P370;    blue-light taxis receptor;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(82)80929-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Halobacterium halobium tactic responses towards light and chemoeffectors are accompanied by changes in the methylation level of methyl-accepting chemotaxis proteins (MCP). Taxis towards green light absorbed by the bacteriorhodopsin proton pump appears to be governed by Δ _H+-sensing. The addition of CCCP, an uncoupler, prevented the increase of MCP methylation in response to green light illumination, but had no effect on CH₃-incorporation followed by the addition of the attractants glucose, leucine and histidine. Similarly, CCCP did not change MCP demethylation in response to blue light illumination, a repelling stimuli.

【 授权许可】

Unknown