Deoxyribose breakdown by the adriamycin semiquinone and H2O2: evidence for hydroxyl radical participation

FEBS Letters

Winterbourn, Christine C.¹  Bates, Diana A.¹ 

[1] Department of Clinical Biochemistry, Christchurch Hospital, Christchurch, New Zealand

关键词: Thiobarbituric acid;    Superoxide dismutase;    Adr_.;    adriamycin semiquinone radical;    TBA;    thiobarbituric acid;    OH_.;    hydroxyl radical;    O2 _.;    superoxide;    DTPA;    diethylenetriamine penta-acetic acid;   

DOI  :  10.1016/0014-5793(82)81222-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report our finding that the reaction between the adriamycin semiquinone (produced by reduction of the drug by xanthine oxidase) and H₂O₂ in N₂ causes deoxyribose degradation to a thiobarbituric acid-reactive chromogen. Deoxyribose breakdown was inhibited by scavengers of hydroxyl radicals, providing evidence for the participation of hydroxyl radicals. The reaction was detected in air, but was less efficient in air than in N₂. Deoxyribose degradation did not require a metal catalyst, and was inhibited by superoxide dismutase in air, but not N₂. A similar reaction with deoxyribose in DNA may be of major importance in the antitumour action of adriamycin.

【 授权许可】

Unknown