Identification of the active site cysteine and of the disulfide bonds in the N‐terminal part of the molecule of bovine spleen cathepsin B

FEBS Letters

Pohl, Jan¹  Kostka, Vladimír¹  Baudyš, Miroslav¹  Tomášek, Vladimír¹ 

[1] Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6, Czechoslovakia

关键词: RAE-;    reduced and aminoethylated-;    dansyl-;    1-dimethylaminonaphthalene-5-sulfonyl-;    DABTH-;    4-N;    N-dimethylaminoazobenzene-4′-thiohydantoin derivative;    PTH-;    phenylthiohydantoin derivative;    HPLC;    high-performance liquid chromatography;    EDTA;    ethylenediamine tetraacetic acid;    DTNB;    5;    5′-dithiobis (2-nitrobenzoic acid);    DTT;    dithiothreitol;    SDS—PAGE;    sodium dodecyl sulfate—polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(82)80210-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 授权许可】

Unknown