VITRIFICATION AFFECTS THE EXPRESSION OF MATRIX METALLOPROTEINASES AND THEIR TISSUE INHIBITORS OF MOUSE OVARIAN TISSUE

Iranian journal of reproductive medicine

ASADZADEH REZA¹  GHORBANIAN MOHAMMAD TAGHI¹  ZAVAREH SAEED¹  KHOSRAVI SHIMA¹  PAYLAKHI SEYED HASSAN¹  MOHEBBI SEYED REZA¹ 

关键词: VITRIFICATION;    PRE ANTRAL FOLLICLES;    MATRIX METALLOPROTEINASES;    MOUSE;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries.Objective: This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified mouse ovaries.Materials and Methods: In this experimental study, the gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the isolated preantral follicles derived from fresh and vitrified ovaries of 14-16 days old female mice through real time qRT-PCR was evaluated. Developmental parameters, including survival rate, growth, antrum formation and metaphase II oocytes were also analyzed.Results: The developmental parameters of fresh preantral follicles were significantly higher than vitrified preantral follicles. The TIMP-1 and MMP-9 expression levels showed no differences between fresh and vitrified preantral follicles (p=0.22, p=0.11 respectively). By contrast, TIMP-2 expression significantly decreased (p=0.00) and MMP-2 expression increased significantly (p=0.00) in vitrified preantral follicles compared with to fresh ones.Conclusion: Changes in expression of MMP-2 and TIMP-2 after ovarian tissues vitrification is partially correlated with decrease in follicle development.

【 授权许可】

Unknown