DERIVATION OF ES-LIKE CELL FROM NEONATAL MOUSE TESTIS CELLS IN AUTOLOGOUS SERTOLI CELLS CO-CULTURE SYSTEM

Iranian journal of reproductive medicine

ASADI MOHAMMAD HOSSEIN¹  JAVANMARDI SETAREH¹  MOVAHEDIN MANSOUREH¹ 

关键词: SPERMATOGONIAL STEM CELLS;    EMBRYONIC STEM LIKE CELL;    PLURIPOTENT STEM CELLS;    CO-CULTURE;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.Objective: This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis.Materials and Methods: Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colony and SSCs respectively.Results: The experiment results indicated that our method of obtaining pluripotent ES-like cells from spermatogonial cells (SCs) is simpler than the described methods. ES-like cells were immunopositive for pluripotency markers. ES-like cell qPCR results indicated significant increase in pluripotency genes expression and significant decrease in germ cell-specific genes expression.Conclusion: The results indicated that ES-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. The pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells.

【 授权许可】

Unknown