EVALUATION OF TWO DNA EXTRACTION METHODS FROM MATERNAL PLASMA FOR USING IN NON-INVASIVE BOVINE FETUS GENDER DETERMINATION

Iranian journal of reproductive medicine

DAVOUDI ARASH¹  SEIGHALANI RAMIN¹  TARANG ALIREZA¹  ALEYASIN SEYED AHMAD¹  SALEHI ABDOLREZA¹  TAHMORESSI FARIDEH¹ 

关键词: DNA EXTRACTION;    MATERNAL PLASMA;    BOVINE FETUS;    GENDER DETERMINATION;    NON-INVASIVE;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: Fetal DNA in maternal plasma and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. Non-invasive prenatal diagnosis is now possible at 8th week of pregnancy, by maternal blood sample testing.Objective: The purpose of this study was to evaluate two DNA extraction methods from mother plasma and its routine clinical application in bovine fetus gender determination with non-invasive method.Materials and Methods: Maternal blood samples were taken from 40 pregnant cows during the 8th-38th weeks of gestation. DNA was extracted from 350

【 授权许可】

Unknown