EFFECT OF POLYUNSATURATED FATTY ACIDS ON SECRETORY PHOSPHOLIPASE A2 TYPE IIA IN ECTOPIC ENDOMETRIAL CELLS

Iranian journal of reproductive medicine

IMANI ALI REZA¹  KHANAKI KOROSH¹  RAHIMIPOUR ALI¹  SOLEIMANI RAD JAFAR¹  MOTEVALIZADE ARDEKANI ALI¹  GHASEMZADEH ALIEH¹  NOURI MOHAMMAD¹  SAREMI ABOTALEB¹  SHAHNAZI VAHIDEH¹  DARABI MASOUD¹  SADEGHI MOHAMMAD REZA¹  MEHDIZADEH AMIR¹ 

关键词: FATTY ACIDS;    ENDOMETRIOSIS;    SECRETORY PHOSPHOLIPASE A2 TYPE IIA;    CELL CULTURE;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: Endometriosis is a common chronic inflammation which leads to infertility and chronic pelvic pain in affected women. Secretory phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is markedly increased in inflammatory disorders.Objective: To assess the effects of �?-3 and �?-6 polyunsaturated fatty acids (PUFAs) administration in endometrial cells culture on sPLA2IIa level and cell survival comparing homolog ectopic versus eutopic endometrial cells from endometriosis patients.Materials and Methods: In this experimental study, ectopic and eutopic endometrial tissue samples obtained from 15 endometriosis patients were immediately frozen. After thawing and tissue digestion, mixed stromal and endometrial gland cells were cultured for 8 days in three different culture media; balanced w-3/w-6, high w-3 and high w-6 PUFAs ratio. Cell survival was measured using 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl) -5- (phenylamino) carbonyl-2H- tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with ELISA technique.Results: The sPLA2IIa level was significantly higher in the ectopic endometrial cell culture compared to the eutopic group for each of the three matched treatments (balanced, high w-3 and high w-6). Also the sPLA2IIa level in the ectopic endometrial cell group was remarkably increased by each of the three PUFAs treatments compared to control condition (p<0.05, p<0.01, p<0.05 respectively). Cell survival in the eutopic group was significantly decreased by high w-6 culturing compared to control medium (p<0.05).Conclusion: The increase in sPLA2IIa level in ectopic endometrial cells by fatty acid treatments (especially high w-3), strengthens the hypothesis that PUFAs stimulate secretion of cytokines leading to increased sPLA2IIa level.

【 授权许可】

Unknown