TESTICULAR BIOHISTOCHEMICAL ALTERATIONS FOLLOWING EXPERIMENTAL VARICOCELE IN RATS

Iranian journal of reproductive medicine

MALEKINEJAD HASSAN¹  SADRKHANLOO RAJAB ALI¹  RAZI MAZDAK¹  SARRAFZADEH REZAEI FARSHID¹ 

关键词: VARICOCELE;    CYTOPLASMIC CARBOHYDRATE;    LIPID FOCI;    LIPASE;    ALKALINE-PHOSPHATES;    TESTIS;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: The exact pathophysiology of testicular degeneration, following varicocele has not been completely understood yet. Objective: The current study was designed to determine the effect of varicocele on germinal epithelium (GE) cytoplasmic biohistochmical alterations.Materials and Methods: To follow-up this study, left varicocele was induced in test groups. Non-varicocelized rats were served as control-sham (n=6). Following 4, 6 and 8 months, right and left testes were dissected out and the blood serum sample was taken. The GE cytoplasmic carbohydrate, lipid accumulation, lipase and alkaline-phosphates (ALP) ratios were analyzed. Serum levels of LH, FSH and testosterone were measured.Results: Observations demonstrated that in varicocele-induced rats, the spermatogenesis cell lineage exhibited lower number of cells with periodic acid shift positive cytoplasm, higher number of cells with lipid and ALP positive stained cytoplasm in comparison to control animals. Lipase enzyme decreased by the time in the test animals. In varicocelized groups the number of Leydig cells decreased in to 2.25

【 授权许可】

Unknown