Iranian journal of reproductive medicine | |
DETERMINATION OF EFFECTIVE DOSAGE OF FSH AND HCG IN THE MATURATION OF PREANTRAL FOLLICLES AND ENCLOSED OOCYTES IN MICE | |
ANVARI M.1  GHANI M.J.1  NAZEM H.A.1  BARZEGARI F.1  JAVED A.1  REZAEI ZARCHI S.1  KALANTAR S.M.1  JAMIL A.1  | |
关键词: FOLLICLE STIMULATING HORMONE; HCG; PREANTRAL FOLLICLES; OOCYTE MATURATION; GVBD; | |
DOI : | |
学科分类:药学、药理学、毒理学(综合) | |
来源: Shahid Sadoughi University of Medical Sciences, Yazd | |
【 摘 要 】
Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM is an intellectual, scientific and clinical challenge with a number of potential applications. Objective: The effect of hCG was evaluated on the timing and regulation of in vitro ovulation for the Syrian mice oocytes in the presence and absence of FSH. Materials and Methods: Preantral follicles, isolated from the ovaries of 6 weeks-old mice, were cultured in TCM-199 medium. The effect of 10-200 mIU/ml FSH and 1.5 IU/ml hCG was seen on the follicle maturation, as well as the changes in ovulation capacity of enclosed oocytes, after the incubation period of 6 days at 37°C, 92% humidity and 5% CO2 in air.Results: 100 mIU/ml FSH showed increased follicle diameter, survival, germinal vesicle breakdown (GVBD) and oocyte maturation rates (p<0.0001). Significantly higher number of follicles showed cumulus attachment when ovulation started within 16-24 hours post hCG (97% and 80% respectively; p<0.0001) as compared to the cultures without hCG or when the ovulation time increased from 24 hours post hCG. Combination of FSH and hCG showed 97% (p<0.0001) ovulation as compared to that seen for FSH-containing medium only (81%) or control (10%).Conclusion: The combined administration of 1.5 IU/ml hCG and 100 mIU/ml FSH induces the in vitro follicle maturation, ovulation capacity and proper timing of mice oocytes.
【 授权许可】
Unknown
【 预 览 】
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RO201912010272871ZK.pdf | 157KB | download |