Iranian journal of reproductive medicine | |
EXPRESSION OF C-JUN IN HUMAN GRANULOSA CELLS FROM PATIENTS PARTICIPATING IN IN VITRO FERTILIZATION PROGRAMS | |
HASANI BAFRANI H.1  SAITO H.1  | |
关键词: APOPTOSIS; C-JUN; GRANULOSA CELLS; IN VITRO FERTILIZATION; HUMAN; | |
DOI : | |
学科分类:药学、药理学、毒理学(综合) | |
来源: Shahid Sadoughi University of Medical Sciences, Yazd | |
【 摘 要 】
Background: The mitogen-activated protein kinase (MAPK) pathway is one of the major signaling pathways that transmit intracellular signals initiated by extracellular stimuli to the nucleus. The stress-activated protein kinase (SAPK)/c-Jun NH2-terminal kinase is a subfamily of MAP kinases implicated in cytokine and stress responses. Objective: In this study, we have examined total and phosphorylated c-Jun in the mural and cumulus granulosa cells, and investigated also whether c-Jun can be responsible for the difference in the expression of apoptosis between mural and cumulus regions.Materials and Methods: A total of 14 consecutive couples participating in IVF program were investigated. Aspirated follicular fluid was transferred into tissue culture dishes and oocyte-cumulus cells complexes were isolated. The cells were centrifuge and fixed with Bouin’s solution and then were put on a glass slide. After fixation, the slides were stained by immunocytochemistry method.The incidence of apoptotic granulosa cells was examined by a fluorescence microscope.Results: The incidence of apoptotic granulosa cells was 1.27±0.12 in the mural region and 0.38±0.07 in the cumulus regions.All mural and cumulus cells expressed total c-Jun in 7 patients while phosphorylated c- Jun was also expressed in all cells of the other 7 patients. There was no difference between apoptotic and nonapoptotic cells in the expression of total and phosphorylated c-Jun.
【 授权许可】
Unknown
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