EXPRESSION OF C-JUN IN HUMAN GRANULOSA CELLS FROM PATIENTS PARTICIPATING IN IN VITRO FERTILIZATION PROGRAMS

Iranian journal of reproductive medicine

HASANI BAFRANI H.¹  SAITO H.¹ 

关键词: APOPTOSIS;    C-JUN;    GRANULOSA CELLS;    IN VITRO FERTILIZATION;    HUMAN;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: The mitogen-activated protein kinase (MAPK) pathway is one of the major signaling pathways that transmit intracellular signals initiated by extracellular stimuli to the nucleus. The stress-activated protein kinase (SAPK)/c-Jun NH2-terminal kinase is a subfamily of MAP kinases implicated in cytokine and stress responses. Objective: In this study, we have examined total and phosphorylated c-Jun in the mural and cumulus granulosa cells, and investigated also whether c-Jun can be responsible for the difference in the expression of apoptosis between mural and cumulus regions.Materials and Methods: A total of 14 consecutive couples participating in IVF program were investigated. Aspirated follicular fluid was transferred into tissue culture dishes and oocyte-cumulus cells complexes were isolated. The cells were centrifuge and fixed with Bouin’s solution and then were put on a glass slide. After fixation, the slides were stained by immunocytochemistry method.The incidence of apoptotic granulosa cells was examined by a fluorescence microscope.Results: The incidence of apoptotic granulosa cells was 1.27±0.12 in the mural region and 0.38±0.07 in the cumulus regions.All mural and cumulus cells expressed total c-Jun in 7 patients while phosphorylated c- Jun was also expressed in all cells of the other 7 patients. There was no difference between apoptotic and nonapoptotic cells in the expression of total and phosphorylated c-Jun.

【 授权许可】

Unknown