THE CHANGES IN MORPHOLOGY AND MORPHOMETRICAL INDICES OF ENDOMETRIUM OF OVARIECTOMIZED MICE IN RESPONSE TO EXOGENOUS OVARIAN HORMONES

Iranian journal of reproductive medicine

FOROUZANDEH MOGHADAM M.¹  REZAZADEH VALOUJERDI M.¹  SALEHNIA M.¹  PEYGHAMBARI F.¹  HAJIZADEH EBRAHIM¹ 

关键词: ENDOMETRIUM;    EXOGENOUS OVARIAN HORMONES;    OVARIECTOMIZED MICE;    MORPHOMETRY;    MORPHOLOGY;   

DOI  :  

学科分类：药学、药理学、毒理学（综合）

来源: Shahid Sadoughi University of Medical Sciences, Yazd

【 摘 要 】

Background: The preparation of endometrium for embryo reception and implantation are controlled by ovarian hormones. These hormones have distinct cyclical changes during estrus cycle.Objective: The aim of this study was to evaluate the changes in morphology and morphometrical indices of endometrium by daily injections of estrogen and progesterone in ovariectomized mouse.Materials and Methods: In total 60 adult NMRI female mice were ovariectomized and after two weeks, they were randomly divided into five groups: control, sham group, estrogen treated mice (which received daily dosage of 0.5 ml/mouse of hormone for five days), progesterone treated mice (which received daily dosage of 0.2 ml/mouse of progesterone hormone for five days) and estrogen-progesterone treated mice (they received 0.5 ml/mouse estrogen on the first day and 0.2 ml/mouse progesterone injections from the second day to the fifth day of treatment). The mice were sacrificed in every day (n=5) up to five days after treatment and their uterine horns were obtained and processed for morphological and morphometrical studies.Results: On the second day of treatment, the diameter of glands was observed to be more in the progesterone group (53.75±6.32m) than this in the estrogen (45.13±7.78 m) and estrogen-progesterone treated groups (48.17±13.58 m). While, the number of glands (76.25±17.37) and thickness of endometrium (39.58±3.37 m) were observed to be more in the estrogen treated group (p=0.01). Conclusion: Progesterone had effect on the gland whereas estrogen caused increased in height of surface epithelium of endometrium. Overall, the day 2 after treatment (in all experimental groups) is suitable day for sampling for further studies.

【 授权许可】

Unknown