Therapeutic Potential of Umbilical Cord Blood Stem Cells on Brain Damage of a Model of Stroke

Journal of Cardiovascular and Thoracic Research

shabnam Mohammadi¹  Hossein Ali Ghafaripoor¹  Darioush Hamidi¹  Mohammad Reza Nikravesh¹  Mehdi Jalali¹  Masoomeh Seghatoleslam¹  Javad Sanchooli¹ 

关键词: Stem Cell;    Stroke;    Brain Damage;    Umbilical Cord Blood;   

DOI  :  10.5681/jcvtr.2011.026

学科分类：农业科学（综合）

来源: Tabriz University of Medical Sciences and Health Services

【 摘 要 】

Introduction: Human cord blood-derived stem cells are a rich source of stem cells as well as precursors. With regard to the researchers have focused on the therapeutic potential of stem cell in the neurological disease such as stroke, the aim of this study was the investiga-tion of the therapeutic effects of human cord blood-derived stem cells in cerebral ischemia on rat. Methods: This study was carried out on young rats. Firstly, to create a laboratory model of ischemic stroke, carotid artery of animals was occluded for 30 minutes. Then, umbilical cord blood cells were isolated and labeled using bromodeoxyuridine and 2×105 cells were injected into the experimental group via the tail vein. Rats with hypoxic condi-tions were used as a sham group. A group of animals did not receive any injection or sur-geries were used as a control. Results: Obtained results were evaluated based on behavior-al responses and immunohistochemistry, with emphasis on areas of putamen and caudate nucleus in the control, sham and experimental groups. Our results indicated that behavioral recovery was observed in the experimental group compared to the either the sham or the control group. However, histological studies demonstrated a low percent of tissue injury in the experimental group in comparison with the sham group. Conclusions: Stem cell trans-plantation is beneficial for the brain tissue reparation after hypoxic ischemic cell death.

【 授权许可】

Unknown