Evaluation of antimicrobial Activities of isolated compounds from the leaf of the white specie of Sesamum Indicum from Benue State, Nigeria

Journal of Applied Sciences and Environmental Management

NDUKWE, GEORGE .I.¹  OGWUCHE, CHRISTIANA .E.¹  AMUPITAN, JOSEPH .O.¹ 

关键词: Anti-microbial;    Benue;    Pathogenic micro-organisms;    Sesamum indicum leaves;    Dry vacuum;    liquid chromatography (DVLC);   

DOI  :  

学科分类：环境科学（综合）

来源: University of Port Harcourt * Department of Pure and Industrial Chemistry

【 摘 要 】

The isolated compounds from the petroleum spirit, chloroform and methanolcrude leaf extracts of the white specie of the leaf of the Sesamum Indicumused by traditionalmedicinal practitioners for the management of infecticious diseases were investigated for invitro antimicrobial activity against some organisms. The various extracts affordedcompounds which had significant antimicrobial activities. The antimicrobial screeningshowed that all the pure isolates from the different solvent extracts were active against theorganisms, Staphylococcus aureus , Streptococcus pyogenes , Salmonella typhi , Candida albicans , Candida krusei , and Candida tropicalisat various MIC’s and (MBC/MFC)’s. Thepure isolates from methanol and ethyl acetate fractions were all inactive against Bacillus cereus , Corynebacterium ulcerans , and Escherichia coli . Only the pure isolates frompetroleum spirit and chloroform fractions showed activity against Bacillus cereus,Corynebacterium ulcerans, and Escherichia coli at an MIC of 0.5mg/ml and MCB/MFC of1mg/ml. Chromatographic techniques such as analytical TLC, Purification by dry vacuumliquid chromatography (DVLC) and Preparative TLC were used.

【 授权许可】

Unknown