Bioremediation of Polycyclic Aromatic Hydrocarbon contaminated Aqueous-Soil matrix: Effect of co-contamination

Journal of Applied Sciences and Environmental Management

Onwuemene, O.C.¹  Owabor, C.N.¹  Enaburekhan, I.¹ 

关键词: Relative toxicity;    Mineralization;    Availability;    Co-contamination;    Activity series;    Complexation;   

DOI  :  

学科分类：环境科学（综合）

来源: University of Port Harcourt * Department of Pure and Industrial Chemistry

【 摘 要 】

This study investigates the effect of lead and chromium on the rate of bioremediation of polycyclicaromatic hydrocarbon (PAH) contaminated clay soil. Naphthalene was used as a target PAH. The soil was sterilizedby heating at 120°C for one hour. 100g of the soil was contaminated with lead, chromium, nickel and mercury (40-200mg/l), 200mg/l of naphthalene and finally inoculated with microbes (Bacillus spp and Aspergillus niger). Acontrol experiment containing these microbes, naphthalene but without heavy metals served was also setup. Residualnaphthalene concentration was taken and analysed every three (3) days. The result of the study showed that lead at40mg/l, 80mg/l, 120mg/l, 160mg/l and 200mg/l had the following percentage degradation 84.45%, 75.68%, 70.23%,60.49%, and 52.95% respectively, Chromium at the same concentrations had 83.89%, 74.95%, 69.99%, 59.95% and52.89%, nickel had 82.49%, 71.89%, 62.86%, 49.89%, 42.34% while mercury had, 73.99%, 71.89%, 48.98%,24.22% and 10.17% at the end of the 42 days contact period. The percent degradation for the control sample wasfound to be 85%. The observed percentages are an indicator that the rate of naphthalene degradation was faster in thecontrol experiment and the results showed that heavy metals inhibited biodegradation of naphthalene by varyingdegrees. At concentrations of 40mg/l-200mg/l of the different heavy metals, there was a steady reduction in thebiodegradation rate, which suggests an increase in the interference with the metabolic activity of the microbes. Thiscan be attributed to the poor colonization of the microbes at these concentrations.

【 授权许可】

Unknown