Ultra-sensitive Determination of Salinomycin in Serum Using ICP-MS with Nanoparticles

Bulletin of the Korean chemical society

H. B. Lim¹  H. K. Cho¹ 

关键词: Bioanalysis;    Antibiotic detection;    Particle tagging;    ICP-MS;    Nanoparticles;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

An ultra-sensitive detection method for small molecules such as antibiotics was developed using ICP-MS with magnetic and TiO2 nanoparticles. Since most of the antibiotics are too small to employ a sandwich-type extraction through an immunoreaction, a non-specific platform was employed, in which the target was extracted by magnetic separation, followed by tagging with TiO2 nanoparticles of 11.2 nm for ICP-MS measurement. The detection limit for salinomycin obtained from spiked serum samples was 0.4 ag mL−1 (± 10.3%), which was about 1.5 × 106 times lower than that of LC-MS/MS and about 1.2 × 1011 times better than that of ELISA. Such an excellent sensitivity enabled us to study the toxicity of antibiotics exposed to human beings by determining them in serum.

【 授权许可】

Unknown