On-Channel Micro-Solid Phase Extraction Bed Based on 1-Dodecanethiol Self-Assembly on Gold-Deposited Colloidal Silica Packing on a Capillary Electrochromatographic Microchip

Bulletin of the Korean chemical society

Shinseon Kim¹  Jongman Park¹ 

关键词: Solid phase extraction;    Capillary electrochromatography;    Microchip colloidal silica packing;    Dodecanethiol self-assembly;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

A fully packed capillary electrochromatographic (CEC) microchip with an on-column micro-solid phase extraction (SPE) bed for the preconcentration and separation of organic analytes was prepared. A linear microchannel with monodisperse colloidal silica packing was formed on a cyclic olefinic copolymer microchip with two reservoirs on both ends. Silver-cemented silica packing frit structure was formed at the entrance of the microchannel by electroless plating treatment as a base layer. A gold coating was formed on it by reducing Au3+ to gold with hydroxylamine. Finally micro-SPE bed was formed by self-assembly adsorption of 1- dodecanethiol on it. Micro-SPE beds were about 100-150 m long. Approximately 103 fold sensitivity enhancements for Sulforhodamine B, and Fluorescein in nM concentration levels were possible with 80 s preconcentration. Basic extraction characteristics were studied.

【 授权许可】

Unknown