Rapid Determination of Imatinib in Human Plasma by Liquid Chromatography- Tandem Mass Spectrometry: Application to a Pharmacokinetic Study

Bulletin of the Korean chemical society

Wooseong Huh¹  Eun Gi Cho¹  Jin Ah Jung¹  Soo-Youn Lee¹  Jae-Wook Ko¹  Jeong Soo Yang¹ 

关键词: Imatinib;    LC-MS/MS;    Plasma;    Validation;    Pharmacokinetic;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

A simple, fast and robust analytical method was developed to determine imatinib in human plasma using liquid chromatography-tandem mass spectrometry with electrospray ionization in the positive ion mode. Imatinib and labeled internal standard were extracted from plasma with a simple protein precipitation. The chromatographic separation was performed using an isocratic elution of mobile phase involving 5.0 mM ammonium formate in water–5.0 mM ammonium formate in methanol (30:70, v/v) over 3.0 min on reversed-stationary phase. The detection was performed using a triple-quadrupole tandem mass spectrometer in multiple-reaction monitoring mode. The developed method was validated with lower limit of quantification of 10 ng/mL. The calibration curve was linear over 10-2000 ng/mL (R2 > 0.99). The method validation parameters met the acceptance criteria. The spiked samples and standard solutions were stable under conditions for storage and handling. The reliable method was successfully applied to real sample analyses and thus a pharmacokinetic study in 27 healthy Korean male volunteers.

【 授权许可】

Unknown