Protein Structural Characterization by Hydrogen/Deuterium Exchange Mass Spectrometry with Top-down Electron Capture Dissociation

Bulletin of the Korean chemical society

Byungjoo Kim¹  Hai Dong Yu¹  Seonghee Ahn¹ 

关键词: FT-ICR MS;    Top-down;    Electron capture dissociation;    Hydrogen deuterium exchange;    Protein structure;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

This study tested the feasibility of observing H/D exchange of intact protein by top-down electron capture dissociation (ECD) mass spectrometry for the investigation of protein structure. Ubiquitin is selected as a model system. Local structural information was obtained from the deuteration levels of c and z. ions generated from ECD. Our results showed that α-helix region has the lowest deuteration level and the C-terminal fraction containing a highly mobile tail has the highest deuteration level, which correlates well with previous X-Ray and HDX/NMR analyses. We studied site-specific H/D exchange kinetics by monitoring H/D exchange rate of several structural motives of ubiquitin. Two hydrogen bonded β-strands showed similar HDX rates. However, the outer β-strand always has higher deuteration level than the inner β-strand. The HDX rate of the turn structure (residues 8-11) is lower than that of β-strands (residues 1-7 and residues 12-17) it connects. Although isotopic distribution gets broader after H/D exchange which results in a limited number of backbone cleavage sites detected, our results demonstrate that this method can provide valuable detailed structural information of proteins. This approach should also be suitable for the structural investigation of other unknown proteins, protein conformational changes, as well as protein-protein interactions and dynamics.

【 授权许可】

Unknown