Covalent Immobilization of Trypsin on a Novel Aldehyde-Terminated PAMAM Dendrimer

Bulletin of the Korean chemical society

Ayuob Aghanejad¹  Soodabeh Davaran¹  Aliasghar Hamidi¹  Davoud Asgari¹  Mohammad R. Rashidi¹ 

关键词: Immobilization;    Trypsin;    Synthesis;    PAMAM dendrimer;    Aldehyde terminated dendrimers;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Dendrimers are a novel class of nonlinear polymers and due to their extensive applications in different fields, called versatile polymers. Polyamidoamine (PAMAM) dendrimers are one of the most important dendrimers that have many applications in nanobiotechnology and industry. Generally aldehyde terminated dendrimers are prepared by activation of amine terminated dendrimers by glutaraldehyde which has two problems, toxicity and possibility of crosslink formation. In this study, novel aldehyde-terminated PAMAM dendrimer was prepared and used for covalent immobilization of trypsin by the aim of finding a special reagent which can prevent crosslinking and deactivation of the enzyme. For this purpose aminoacetaldehydedimethylacetal (AADA) was used as spacer group between aldehyde-terminated PAMAM and trypsin.The findings of this study showed that immobilization of trypsin not only resulted higher optimal temperature, but also increased the thermal stability of the immobilized enzyme in comparison to the free enzyme.

【 授权许可】

Unknown