Sequence Coverage Enhancement Using Magnetic Nanoparticles in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Protein Analysis

Bulletin of the Korean chemical society

Hie-Joon Kim¹  Jin-Su Song¹  Eunhye Park¹ 

关键词: Matrix-assisted laser desorption/ionization (MALDI);    Sequence coverage;    Magnetic nanoparticles (MNPs);    Phosphoric acid;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Magnetic nanoparticles (MNPs) treated with phosphoric acid were used to improve sequence coverage in protein identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Sample solution of tryptic peptides from proteins was mixed with the MNPs, and the MNPs were separated from the supernatant using a magnet. MALDI mass spectra obtained separately from the supernatant and the MNPs were distinctly different and complementary to each other. Combination of the two spectra led to a significantly increased sequence coverage.

【 授权许可】

Unknown