Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling

Bulletin of the Korean chemical society

Meili Hu¹  Yan Chen¹  Mingxing Jiang¹  Guiqing Kai¹  Ruijun Wang¹  Huali Cui¹ 

关键词: CdSe quantum dots;    Carbohydrates molecules;    HepG2 mannose receptor;    Receptor-mediated endocytosis;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)- coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.

【 授权许可】

Unknown