Rapid Determination of Volatile Organic Compounds in Human Whole Blood Using Static Headspace Sampling with Gas Chromatography and Mass Spectrometry

Bulletin of the Korean chemical society

Ji-Young Lee¹  Heesoo Pyo¹  Jeongae Lee¹  Jong-Ho Choi¹  Jong-Tae Lee¹  Seungki Kim¹ 

关键词: VOCs;    Whole blood;    Headspace;    HS-GC-MS;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at 60 °C, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng mL−1 and 0.05-0.5 ng mL−1. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng mL−1; detected levels of benzene were 0.05-2.20 ng mL−1.

【 授权许可】

Unknown