Interaction of Bilobalide and Ginkgolides B with Bovine Serum Albumin: A Fluorescence Quenching Study

Bulletin of the Korean chemical society

Shusheng Wang¹  Yan Chen¹  Yi Yang¹  Shaofei Li¹  Guiqing Kai¹  Ruijun Wang¹ 

关键词: Bilobalide;    Ginkgolides B;    Bovine serum albumin;    Fluorescence spectroscopy;    Interaction;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

The interaction of bilobalide (BB) and ginkgolides B (GB) with bovine serum albumin (BSA) was investigated by fluorescent technique and UV/vis absorption spectroscopy. The results showed that BB and GB could intensively quench the fluorescence of BSA through a static quenching procedure. The binding constants (Ka) and the average binding distance between the donor (BSA) and the acceptor (ginkgolides) were obtained (rBB = 5.33 nm and rGB = 4.20 nm) by the theory of non-radiation energy transfer, and then the thermodynamic parameters such as ΔS0 (0.17-0.32 kJ/mol), ΔG0 (-20.76 ~ -17.79 kJ/mol) and ΔH0 (32.47-76.52 kJ/mol) could be calculated, respectively. All these results revealed that the interaction of BB and GB with BSA were driven mainly by hydrophobie force. The synchronous fluorescence spectroscopy was applied to examine the effect of two ginkgolides on the configuration of BSA. The configuration alteration of BSA could be induced by the hydrophobicitv environment of tyrosine with the increase of the drug concentration.

【 授权许可】

Unknown