Graphite Furnace Atomic Absorption Spectrophotometric Determination of Trace Horseradish Peroxidase Using Nanosilver

Bulletin of the Korean chemical society

Wei Lin¹  Jiang Zhi-liang¹  Tang Ya-fang¹  Liang Ai-hui¹ 

关键词: Horseradish peroxidase;    Nanosilver;    Graphite furnace atomic absorption spectrophotometry;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In pH 4.2 HAc-NaAc buffer solution, horseradish peroxidase (HRP) catalyzed H2O2 oxidation of nanosilver to form Ag+. After centrifugation, Ag+ in the supernatant can be measured by graphite furnace atomic absorption spectrophotometry (GFAAS) at the silver absorption wavelength of 328.1 nm. When HRP concentration increased, the Ag+ concentration in the supernatant increased, and the absorption value enhanced. The HRP concentration in the range of 0.84-50 ng ·mL−1 was linear to the enhanced absorption value (ΔA), with a regression equation of ΔA=0.012C+0.11, correlation coefficient of 0.9988, and detection limit of 0.41 ng·mL−1 HRP. The proposed GFAAS method was used to detect HRP in waste water samples, with satisfactory results.

【 授权许可】

Unknown