Characterization of Two Ricin Isoforms by Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis and Capillary Isoelectric Focusing

Bulletin of the Korean chemical society

Byung Ho Woo¹  Kang Choon Lee¹  Myung Sun Kim¹  Cheong Kwan Cho¹  Hye Suk Lee¹  Dong Hee Na¹  Eun Ji Park¹ 

关键词: Ricin;    Capillary electrophoresis;    Capillary isoelectric focusing;    Sodium dodecyl sulfate-capillary gel electrophoresis;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) and capillary isoelectric focusing (CIEF) were employed to characterize and compare ricin E purified from the small grain seeds of Ricinus communis with ricin D isoform. During the purification of ricin E using ion-exchange and size-exclusion chromatography, SDS-CGE was found to be useful for monitoring the efficiencies of purification steps. SDS-CGE showed that the molecular size of ricin E was not significantly different from that of ricin D, which was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. CIEF was useful for discriminating ricin E from ricin D based on their isoelectric points (pI). The pI values of ricin E and D were 8.6-8.8 and 7.0-7.4, respectively. This study demonstrates the usefulness of SDS-CGE and CIEF for the characterization of ricin toxins.

【 授权许可】

Unknown