Electronic Detection of Biomarkers by Si Field-Effect Transistor from Undiluted Sample Solutions with High Ionic Strengths

Bulletin of the Korean chemical society

Chil Seong Ah¹  Chan Woo Park¹  Ansoon Kim¹  Chang Geun Ahn¹  Tae Youb Kim¹  Jong Heon Yang¹  Gun Yong Sung¹  Wan Joong Kim¹  In Bok Baek¹ 

关键词: Electronic detection;    PSA;    Si field effect transistor (FET);    Ionic strength;    Real-time;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In this study, we have developed a new detection method using Si field effect transistor (FET)-type biosensors, which enables the direct monitoring of antigen-antibody binding within very high-ionic-strength solutions such as 1】PBS and human serum. In the new method, as no additional dilution or desalting processes are required, the FET-type biosensors can be more suitable for ultrasensitive and real-time analysis of raw sample solutions. The new detection scheme is based on the observation that the strength of antigen-antibody-specific binding is significantly influenced by the ionic strength of the reaction solutions. For a prostate specific antigen (PSA), in some conditions, the binding reaction between PSA and anti-PSA in a low-ionic strength reaction solution such as 10 レM phosphate buffer is weak (reversible), while that in high-ionic strength reaction solutions such as 1】PBS or human serum is strong.

【 授权许可】

Unknown