Liquid Crystal-based Imaging of Biomolecular Interactions at Roller Printed Protein Surfaces

Bulletin of the Korean chemical society

Min Kyung Park¹  Chang Hyun Jang¹ 

关键词: Liquid crystals;    4-Cyano-4¡¯-pentylbiphenyl (5CB);    Orientation of 5CB;    Roller printing;    Protein-receptor complexation;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In this study, the orientational behavior of thermotropic liquid crystals (LC) supported on a film of protein receptors was examined. Avidin was roller printed and covalently immobilized onto the surface of gold using NHS/EDC chemistry. The orientation of nematic 4-cyano-4�?-pentylbiphenyl (5CB) was found to be parallel to the plane of the printed avidin surface before incubation with a solution of biotin. However, protein-receptor complexation induced a random orientation of 5CB, where protein-receptor complexes disturbed the nanoscale topography of the printed protein surface. Atomic force microscopy and ellipsometry was used to confirm printing and the specific interaction of proteins. These results demonstrate that the combination of LC and roller printing can be used to detect specific interactions between biomolecules by manipulating the orientational behavior of LC to the printed protein surfaces.

【 授权许可】

Unknown