Identification of dinitrotoluene selective peptides by phage display cloning

Bulletin of the Korean chemical society

Won Kyu Lee¹  Seok Chan Kim¹  Jung Hyun Na¹  Hyun Jin Jung¹  Si Hyung Lim¹  Woong Hee Lee¹  Yeon Gyu Yu¹  Bong Suk Jin¹  Hyeon Jun Jang¹ 

关键词: Explosive;    DNT;    Sensor;    Peptide;    Phage display;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Biomolecules specific to explosives can be exploited as chemical sensors. Peptides specific to immobilized dinitrotoluene (DNT) were identified using a phage display library. A derivative of DNT that contained an extended amine group, 4-(2,4-dinitrophenyl)butan-1-amine, was synthesized and immobilized using a self-assembled monolayer surface on gold. Filamentous M13 phages displaying random sequences of 12-mer peptides specific to the immobilized DNTderivate were isolated from the M13 phage library by biopanning. A common peptide sequence was identified from the isolated phages and the synthesized peptides showed selective binding to DNT. When the peptide was immobilized on a quartz crystal microbalance (QCM) chip, it showed a binding signal to DNT, while toluene barely showed significant binding to the QCM chip. These results demonstrate that peptides screened by biopanning against immobilized DNT can be useful for quick and accurate detection of DNT.

【 授权许可】

Unknown