A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins

Bulletin of the Korean chemical society

Doo Wan Boo¹  Soo Jin Oh¹  Chanhoo Park¹  Yeon Gyu Yu¹  Yun Kyoung Kim¹  Bong Suk Jin¹  Hyesung Jeon¹ 

关键词: HBV;    PreS;    Capsid;    Interaction;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain of PLC-�?. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for screening compounds that may potentially inhibit or prevent the assembly process of HBV particles.

【 授权许可】

Unknown