Electron Caption Dissociation of Proteins Initiated by Photoelectrons Generated from 266 nm UV Laser Radiation on an ICR Cell Wall

Bulletin of the Korean chemical society

Wha Keun Ahn¹  Bum Ku Rhee¹  Han Bin Oh¹  Sun Young Lee¹  Yewon Lee¹  Hyuk Kang¹  Ki Ho Cho¹  Soojin Park¹ 

关键词: 266 nm UV laser;    Electron capture dissociation (ECD);    Ubiquitin;    Azurin;    FTICR MS;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

It was demonstrated that ECD MS can be successfully applied to Bovine ubiquitin (8.6 kDa, 76 amino acids) and Pseudomonas aeruginosa azurin (13.9 kDa, 128 amino acids, 1 disulfide bond) cations using photoelectrons emitted by irradiating a 266 nm UV laser light on the edge of an ion cyclotron resonance (ICR) cell trapping plate hole. The photoelectron ECD method cleaved protein backbone bonds as effectively as conventional ECD MS methods that employed a tungsten (W) wire or heated dispenser cathode as an electron source. This method offers the advantage of providing electrons for ECD MS without an apparent electron source.

【 授权许可】

Unknown