Expression of Acetohydroxyacid Synthase from Bacillus anthracis and Its Potent Inhibitors

Bulletin of the Korean chemical society

Sung-Hwan Han¹  Jinheung Kim¹  Kyoung-Jae Choi¹  Moon-Young Yoon¹  Chien Ngoc Pham¹  Hoeil Jung¹  Jung-Do Choi¹ 

关键词: Acetohydroxyacid synthase;    Herbicides;    Resistance;    Kinetic parameters;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Acetohydroxyacid synthase (AHAS, EC 2. 2. 1. 6) is the enzyme that catalyses the first step in the common pathway of the biosynthesis of the branched chain amino acids, valine, leucine and isoleucine. For the first time, the AHAS gene from Bacillus anthracis was cloned into the expression vector pET28a(+), and was expressed in the E. coli strain BL21(DE3). The purified enzyme was checked on 12% SDS-PAGE to be a single band with molecular weight of 65 kDa. The optimum pH and temperature for B. anthracis AHAS was at pH 7.5 and 37 oC, respectively. Kinetic parameters of B. anthracis were as follows: Km for pyruvate, K0.5 for ThDP and Mg2+ was 4.8, 0.28 and 1.16 mM respectively. AHAS from B. anthracis showed strong resistance to three classes of herbicides, Londax (a sulfonylurea), Cadre (an imidazolinone), and TP (a triazolopyrimidine). These results indicated that these herbicides could be used in the search for new anti-bacterial drugs.

【 授权许可】

Unknown