Oxidative Modification of Neurofilament-L by the Cytochrome c and Hydrogen Peroxide System

Bulletin of the Korean chemical society

Jung Hoon Kang¹ 

关键词: Neurofilament-L;    Cytochrome c;    Oxidative stress;    Parkinson¡¯s disease;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

As neurofilament proteins are major cytoskeletal components of neuron, abnormality of neurofilament is proposed in brain with neurodegenerative disorders such as Parkinson’s disease (PD). Since oxidative stress might play a critical role in altering normal brain proteins, we investigated the oxidative modification of neurofilament-L (NF-L) induced by the reaction of cytochrome c with H₂O₂. When NF-L was incubated with cytochrome c and H₂O₂, the protein aggregation was increased in cytochrome c and H₂O₂ concentrationsdependent manner. Radical scavengers, azide, formate and N-acetyl cysteine, prevented the aggregation of NFL induced by the cytochrome c/H₂O₂ system. The formations of carbonyl group and dityrosine were obtained in cytochrome c/H₂O₂-mediated NF-L aggregates. Iron specific chelator, desferoxamine, prevented the cytochrome c/H₂O₂ system-mediated NF-L aggregation. These results suggest that the cytochrome c/H₂O₂ system may be related to abnormal aggregation of NF-L which may be involved in the pathogenesis of PD and related disorders.

【 授权许可】

Unknown