Aggregation of ¥á-Synuclein Induced by Oxidized Catecholamines as a Potential Mechanism of Lewy Body

Bulletin of the Korean chemical society

Kyung Sik Kim¹  Jung Hoon Kang¹ 

关键词: Lewy body;    ¥á-Synuclein;    L-DOPA;    Carnosine;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Lewy bodies (LBs) are neuronal inclusions that are closely related to Parkinson`s disease (PD). The filamentous component of LB from patients with PD contains biochemically altered �?-synuclein. We have investigated the effect of the oxidized products of catecholamines on the modification of �?-synuclein. When �?-synuclein was incubated with the oxidized 3,4-dihydroxyphenylalanine (L-DOPA) or dopamine, the protein was induced to be aggregated. The oxidized catecholamine-mediated �?-synuclein aggregation was enhanced by copper ion. Radical scavengers, azide and N-acetyl cysteine significantly prevented the oxidized catecholamine-mediated �?-synuclein aggregation. The results suggest that free radical may play a role in �?-synuclein aggregation. Exposure of �?-synuclein to the oxidized products of catecholamines led to the formation of dityrosine. Antioxidant dipeptides carnosine, homocarnosine and anserine significantly protected �?-synuclein from the aggregation induced by the oxidized products of catecholamines.

【 授权许可】

Unknown