Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip

Bulletin of the Korean chemical society

Dae-Hee Park¹  Kyung-Sup Lee¹  Yong-Sung Choi¹ 

关键词: Indicator-free DNA chip;    Immobilization;    Target DNA;    Hybridization;    Ferricyanide;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

This research aims to develop DNA chip array without an indicator. We fabricated microelectrode array by photolithography technology. Several DNA probes were immobilized on an electrode. Then, indicator-free target DNA was hybridized by an electrical force and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between DNA probe and mismatched DNA in an anodic peak. Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent. This indicator-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from 10-18 M to 10-5 M in the buffer solution. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.

【 授权许可】

Unknown