Integrated Microdisk Gold Electrode Modified with Metal-porphyrin and Metal-phthalocyanines for Nitric Oxide Determination in Biological Media

Bulletin of the Korean chemical society

Hyun Ja Chun¹  Hyun Ok Bae¹  Il Kwang Kim¹  Young Jin Kim¹  Gi Soo Oh¹  Hun Taeg Chung¹ 

关键词: Modified electrode;    NO determination;    Metal-phthalocyanine;    Square wave anodic stripping voltammetry;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

An integrated gold microdisk electrode was constructed and modified with metal-porphyrin or metalphthalocyanines for NO determination in biological media. Microanalysis of NO using square wave anodic stripping voltammetry in 1 �? 10^-2 M HClO₄ was optimal when the accumulation potential was 0.1V, frequency 100Hz, and the scan rate was 200 mV/s. When the electrode was modified with metal-porphyrin or metalphthalocyanines, the anodic peak currents of NO increased due to the catalytic oxidation of NO. In case of Fe(II)-phthalocyanine modified electrode, the peak currents remarkably increased and the sensitivity was high. The calibration curve had good linearity in the range from 3.6 �? 10^-5 M to 7.2 �? 10^-7 M, and the detection limit was 5.7 �? 10^-7 M. For the structural stability and increased sensitivity, Fe(II)-phthalocyanine modified gold microdisk electrode coated with Nafion was applied to determination of NO released from cultured macrophase.

【 授权许可】

Unknown