Production and Characterization of Monoclonal Antibodies to a Generic Hapten for Class-Specific Determination of Organophosphorus Pesticides

Bulletin of the Korean chemical society

Choon Sup Ra¹  Hyuk-Man Kwon¹  Soo Jung Lee¹  Xiaoping Xue¹  Mi Soon Jang¹  Yong Tae Lee¹  Taeowan Chung¹ 

关键词: Organophosphorus pesticides;    Class-specific determination;    Enzyme-linked immunosorbent assay;    ELISA;    Monoclonal antibodies;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Monoclonal antibodies have been generated against a generic hapten, �?,�?-diethyl �?-(5-carboxy-2-fluorophenyl) phosphorothioate, for the determination of organophosphorus (OP) pesticides in a class-specific manner. In an indirect competitive enzyme-linked immunosorbent assay (ELISA) format, employing a heterologous coating antigen, these monoclonal antibodies showed desirable properties for use in the class-specific determination, i.e., broad specificity and high sensitivity. The IC₅₀ values of four commonly used �?,�?-diethyl OP pesticides were fairly uniform ranging from 0.1 to 0.3 Л/mL. The IC₅₀ values of three �?,�?-dimethyl derivatives were between 0.3 and 1.4 Л/mL. These values, together with the limits of detection (LOD), were better, in terms of the specificity and sensitivity, compared with the values obtained previously with polyclonal antibodies.

【 授权许可】

Unknown