Development of an ELISA for the Organophosphorus Insecticide Isofenphos

Bulletin of the Korean chemical society

Won Chul Park¹  Choon Sup Rha¹  Yong Tae Lee¹  Han Jin Park¹  Taeowan Jung¹ 

关键词: Isofenphos;    Insecticide;    Enzyme-linked immunosorbent assay;    ELISA.;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

(R,S)- and (R)-2-Benzylcysteine (1) and (R,S)-2-phenethylcysteine (2) were synthesized and evaluated as inhibitors for carboxypeptidase A (CPA) with the expectation that these compounds exhibit improved inhibitory activities over 2-benzyl-3-mercaptopropanoic acid (BMPA), a potent CPA competitive inhibitor, possibly having additional interactions of their amino group with the carboxylate of Glu-270 of the enzyme upon binding to CPA. Contrary to the expectation, however, the CPA inhibitory potencies of these compounds were found to be much reduced compared with that of BMPA, suggesting that the amino group in the inhibitors rather exerts steric hindrance in binding of these inhibitors to CPA.

【 授权许可】

Unknown