Phytosynthesized iron nanoparticles: effects on fermentative hydrogen production by Enterobacter cloacae DH-89

Bulletin of materials science

Shymal Kumar Das²  Manabendra Mandal¹  Dhrubajyoti Nath¹  Kuldeep Gupta¹  Devabrata Saikia¹  Ajay Kumar Manhar¹ 

[1] Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784 028, Assam, India$$Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784 028, Assam, IndiaDepartment of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur 784 028, Assam, India$$;Department of Physics, Tezpur University, Napaam, Tezpur 784 028, Assam, India$$Department of Physics, Tezpur University, Napaam, Tezpur 784 028, Assam, IndiaDepartment of Physics, Tezpur University, Napaam, Tezpur 784 028, Assam, India$$

关键词: Iron nanoparticles;    green synthesis;    Syzygium cumini;    dark fermentation;    biohydrogen production;    Enterobacter cloacae.;   

DOI  :  

学科分类：材料工程

来源: Indian Academy of Sciences

【 摘 要 】

In recent years the application of metal nanoparticles is gaining attention in various fields. The present study focuses on the additive effect of `green’ synthesized iron nanoparticles (FeNPs) on dark fermentative hydrogen (H₂) production by a mesophilic soil bacterium Enterobacter cloacae. The FeNPs were synthesized by a rapid green method from FeSO₄ using aqueous leaf extract of Syzygium cumini. The synthesized FeNPs showed a characteristic surface plasmon resonance peak at 267 nm. The transmission electron microscopy images confirm that the formation of FeNPs was mainly porous and irregular in shape, with an average particle size of 20â€�??25 nm. The presence of iron (Fe) in the synthesized FeNPs was confirmed by energy-dispersive X-ray spectroscopy. The comparative effect of FeSO₄ and FeNPs on batch fermentative H₂ production from glucose was investigated. The fermentation experiments reveal that the percentage and yield of H₂ in FeNPs supplementation were increased significantly than the control (no supplementation) and FeSO₄ containing media. The maximum H₂ yield of 1.9 mol mol^âˆ�?1 glucose utilized was observed in 100 mg l^âˆ�?1 FeNPs supplementation, with two-fold increase in glucose conversion efficiency. Thus, the result suggests that FeNPs supplementation in place of FeSO₄ could improve the bioactivity of H₂ producing microbes for enhanced H₂ yield and glucose consumption.

【 授权许可】

Unknown