Ionic liquid modified carbon paste electrode and investigation of its electrocatalytic activity to hydrogen peroxide

Bulletin of materials science

Erol Akyilmaz¹  Erhan Canbay¹  Hayati Türkmen² 

[1] Department of Biochemistry, Faculty of Science, Ege University 35100 Bornova-Izmir/Turkey$$Department of Biochemistry, Faculty of Science, Ege University 35100 Bornova-Izmir/TurkeyDepartment of Biochemistry, Faculty of Science, Ege University 35100 Bornova-Izmir/Turkey$$;Department of Chemistry, Faculty of Science, Ege University 35100 Bornova-Izmir/Turkey$$Department of Chemistry, Faculty of Science, Ege University 35100 Bornova-Izmir/TurkeyDepartment of Chemistry, Faculty of Science, Ege University 35100 Bornova-Izmir/Turkey$$

关键词: Biosensor;    ionic liquid;    1-methyl-3-butylimidazolium bromide;    carbon paste electrode;    horseradish peroxidase.;   

DOI  :  

学科分类：材料工程

来源: Indian Academy of Sciences

【 摘 要 】

This paper reports on the preparation and advantages of novel amperometric biosensors in the presence of hydrophobic ionic liquid (IL), 1-methyl-3-butylimidazolium bromide ([MBIB]). Carbon paste bio-sensor has been constructed by entrapping horseradish peroxidase in graphite and IL mixed with paraffin oil as a binder. The resulting IL/graphite material brings new capabilities for electrochemical devices by combining the advantages of ILs composite electrodes. Amounts of H₂O₂ were amperometrically detected by monitoring current values at reduction potential (â€�??0.15 V) of K₃Fe(CN)₆. Decrease in biosensor responses were linearly related to H₂O₂ concentrations between 10 and 100 𝜇M with 2 s response time. Limit of detection of the biosensor were calculated to be 3.98 𝜇M for H₂O₂. In the optimization studies of the biosensor some parameters such as optimum pH, optimum temperature, enzyme amount, interference effects of some substances on the biosensor response, reproducibility and storage stability were carried out. The promising results are ascribed to the use of an ionic liquid, which forms an excellent charge-transfer bridge and wide electrochemical windows in the bulk of carbon paste electrode.

【 授权许可】

Unknown