Evolutionary Recovery of a Recombinant Viral Genome

G3: Genes, Genomes, Genetics

Ian J. Molineux⁴  James J. Bull²  Devanshi S. Kapadia-Desai³  Rachael Springman¹ 

[1] Center for Computational Biology and BioinformaticsCenter for Computational Biology and BioinformaticsCenter for Computational Biology and Bioinformatics;Center for Computational Biology and BioinformaticsSection of Integrative BiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Center for Computational Biology and BioinformaticsCenter for Computational Biology and BioinformaticsSection of Integrative BiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Section of Integrative BiologyCenter for Computational Biology and BioinformaticsSection of Integrative BiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Institute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Center for Computational Biology and BioinformaticsSection of Integrative BiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712;Section of Integrative BiologySection of Integrative BiologySection of Integrative Biology;Section of Molecular Genetics and MicrobiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Section of Molecular Genetics and MicrobiologySection of Molecular Genetics and MicrobiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Institute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712Section of Molecular Genetics and MicrobiologyInstitute for Cellular and Molecular Biology, The University of Texas at Austin, Texas 78712

关键词: genome;    experimental evolution;    adaptation;    compensatory evolution;    incompatibility;    engineering;   

DOI  :  10.1534/g3.112.002758

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

It is well appreciated that the evolutionary divergence of genes and genomes from a common ancestor ultimately leads to incompatibilities if those genomes are hybridized. Far less is known about the ability and nature of compensatory evolution to yield the recovery of function in hybrid genomes. Here the major capsid gene of the bacteriophage T7 (40-kb dsDNA) was replaced with the homologous gene of either T3 or K11, each 22% different at the protein level from the T7 homolog. Initial fitness was moderately impaired for the T3 exchange, but the K11 exchange was not viable without a compensatory change in the T7 scaffolding protein. Subsequent adaptation of the transgenic phages led to nearly complete fitness recoveries. Compensatory changes were few, mostly in the transgene and its main interacting partner, the scaffolding protein gene. The large magnitude of fitness recovery with relatively few mutations suggests that the fitness costs of hybridizations and horizontal gene exchanges between moderately diverged genomes can potentially be short-lived through compensatory evolution.

【 授权许可】

Unknown