Simplified Insertion of Transgenes Onto Balancer Chromosomes via Recombinase-Mediated Cassette Exchange

G3: Genes, Genomes, Genetics

Jack R. Bateman¹  Florence F. Sun¹  Martin P. Zeidler²  Justine E. Johnson¹ 

[1] Biology Department, Bowdoin College, Brunswick, Maine 04011Biology Department, Bowdoin College, Brunswick, Maine 04011Biology Department, Bowdoin College, Brunswick, Maine 04011;MRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United KingdomMRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United KingdomMRC Centre for Developmental and Biomedical Genetics, University of Sheffield, Sheffield S10 2TN, United Kingdom

关键词: RMCE;    targeted transgenesis;    phiC31;    Drosophila;    balancer;   

DOI  :  10.1534/g3.112.002097

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE).

【 授权许可】

Unknown