Genetic Interactions Between Brassinosteroid-Inactivating P450s and Photomorphogenic Photoreceptors in Arabidopsis thaliana

G3: Genes, Genomes, Genetics

Michael M. Neff²  Kulbir Singh Sandhu²  Katherine Hagely¹ 

[1] College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri 65211College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri 65211College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri 65211;Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164

关键词: CYP734A1;    CYP72C1;    phytochrome A;    phytochrome B;    cryptochrome 1;   

DOI  :  10.1534/g3.112.004580

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Plants use light as a source of information via a suite of photomorphogenic photoreceptors to optimize growth in response to their light environment. Growth-promoting hormones such as brassinosteroids also can modulate many of these responses. BAS1 and SOB7 are brassinosteroid-catabolizing P450s in Arabidopsis thaliana that synergistically/redundantly modulate photomorphogenic traits such as flowering time. The role of BAS1 and SOB7 in photomorphogenesis has been investigated by studying null-mutant genetic interactions with the photoreceptors phyA, phyB, and cry1 with regard to seed germination and flowering time. The removal of BAS1 and/or SOB7 rescued the low germination rate of the phyA-211 phyB-9 double-null mutant. With regard to floral induction, bas1-2 and sob7-1 showed a complex set of genetic interactions with photoreceptor-null mutants. Histochemical analysis of transgenic plants harboring BAS1:BAS1-GUS and SOB7:SOB7-GUS translational fusions under the control of their endogenous promoters revealed overlapping and distinct expression patterns. BAS1’s expression in the shoot apex increases during the phase transition from short-to-long-day growth conditions and requires phyB in red light. In summary, BAS1 and SOB7 displayed both simple and complex genetic interactions with the phytochromes in a plant-stage specific manner.

【 授权许可】

Unknown