Mixed Model Association Mapping for Fusarium Head Blight Resistance in Tunisian-Derived Durum Wheat Populations

G3: Genes, Genomes, Genetics

Elias M. Elias³  Mehdi Sargolzaei¹  Mohamed Mergoum³  Tika Adhikari²  Omid Ansari⁴  Shahryar F. Kianian³  Sujan Mamidi³  Farhad Ghavami³ 

[1] Center for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, CCenter for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, CCenter for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, C;Department of Plant Pathology, North Dakota State University, Fargo, North Dakota 58108Department of Plant Pathology, North Dakota State University, Fargo, North Dakota 58108Department of Plant Pathology, North Dakota State University, Fargo, North Dakota 58108;Department of Plant SciencesDepartment of Plant SciencesDepartment of Plant Sciences;Grains Research and Development Corporation (GRDC), Barton, Australian Capital Territory 2600, AustraliGrains Research and Development Corporation (GRDC), Barton, Australian Capital Territory 2600, AustraliGrains Research and Development Corporation (GRDC), Barton, Australian Capital Territory 2600, Australi

关键词: association mapping;    durum wheat;    Fusarium head blight;    QTL analysis;    suppressor of resistance;   

DOI  :  10.1534/g3.111.000489

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Sources of resistance to Fusarium head blight (FHB) in wheat are mostly restricted to Chinese hexaploid genotypes. The effort to incorporate the resistance from hexaploid wheat or wild relatives to cultivated durum wheat (Triticum turgidum L. var. durum Desf.) have not been successful in providing resistance to the level of the donor parents. In this study, we used 171 BC₁F₆ and 169 BC₁F₇ lines derived from crossing of four Tunisian tetraploid sources of resistance (Tun7, Tun18, Tun34, Tun36) with durum cultivars ‘Ben,’ ‘Maier,’ ‘Lebsock,’ and ‘Mountrail’ for association studies. The Tun18 and Tun7 FHB resistances were found to be comparable to the best hexaploid wheat sources. A new significant QTL for FHB resistance was identified on the long arm of chromosome 5B (Qfhs.ndsu-5BL) with both association and classical QTL mapping analysis. Linkage disequilibrium (LD) blocks extending up to 40 cM were evident in these populations. The linear mixed model considering the structure (Q or P) and the kinship matrix (K_T) estimated by restricted maximum likelihood (REML) was identified as the best for association studies in a mixture of wheat populations from a breeding program. The results of association mapping analysis also demonstrated a region on the short arm of chromosome 3B as potentially linked to FHB resistance. This region is in proximity of major FHB resistance gene fhb1 reported in hexaploid wheat. A possibility of having susceptibility or suppressor of resistance gene(s) on durum wheat chromosome 2A was further confirmed in this material, explaining the problem in developing resistant genotypes without counter selection against this region.

【 授权许可】

Unknown