Nanoparticles Modified by Encapsulation of Ligands with a Long Alkyl Chain to Affect Multispecific and Multimodal Imaging

Journal of Nuclear Medicine

Lathika Hoigebazar¹  Dong Soo Lee¹  Yun-Sang Lee¹  Myung Chul Lee¹  June-Key Chung¹  Hyewon Youn¹  Bo Yeun Yang¹  Byung Chul Lee¹  Young Kyoung Lee¹  Jae Min Jeong¹ 

关键词: nanocrystal;    amphiphile;    Tween 60;    mannose;    lactose;    Q-dot;    RGD;   

DOI  :  10.2967/jnumed.111.092759

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

The attachment of specific ligands to the surfaces of nanoparticles is important for medical and biologic imaging. However, covalent modification of nanoparticles has inherent problems in reproducibility because of many factors such as temperature, pH, concentration, and reaction time. Thus, we developed a method for modifying nanoparticles by encapsulation with specific ligand-conjugated amphiphiles. Methods: We synthesized special amphiphiles with a hydrophilic head and a long single-alkyl chain, such as arginine-glycine-aspartic acid-C18, mannose-C18, lactose-C18, and 2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid-C18. And then we produced stable quantum dots (QDs) encapsulated with polysorbate 60 (a branched polyethylene glycol head with a C18 tail) and the synthesized special amphiphiles. The prepared encapsulated QDs were subject to in vitro and in vivo animal biodistribution studies and small-animal PET studies to confirm their specific binding. Results: The encapsulated QDs could specifically bind to target cells in vitro and in vivo and could be labeled with 68Ga (a positron emitter) easily and with high efficiency. Conclusion: The nanoparticles encapsulated with special amphiphiles could provide a straightforward and novel imaging solution with multimodality and multispecificity.

【 授权许可】

Unknown