Uptake of 11C-Choline in Mouse Atherosclerotic Plaques

Journal of Nuclear Medicine

V. Jukka O. Laine¹  Anne Roivainen¹  Päivi M. Marjamäki¹  Kjell Någren¹  Johanna M.U. Silvola¹  Iina E.K. Laitinen¹  Pauliina Luoto¹  Sanna Hellberg¹  Juhani Knuuti¹  Seppo Ylä-Herttuala¹ 

关键词: atherosclerosis;    autoradiography;    biodistribution;    cardiology;    molecular imaging;    ¹¹C-choline;   

DOI  :  10.2967/jnumed.109.071704

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

The purpose of this study was to explore the feasibility of 11C-choline in the assessment of the degree of inflammation in atherosclerotic plaques. Methods: Uptake of 11C-choline was studied ex vivo in tissue samples and aortic sections excised from 6 atherosclerotic mice deficient for both low-density lipoprotein receptor and apolipoprotein B48 (LDLR−/−ApoB100/100) and 5 control mice. The autoradiographs were compared with the immunohistology of the arterial sites. Results: The uptake of 11C-choline (percentage of the injected activity per gram of tissue) in the atherosclerotic aortas of the LDLR−/−ApoB100/100 mice was significantly higher (1.9-fold, P = 0.0016) than that in the aortas of the control mice. The autoradiography analysis showed significantly higher uptake of 11C-choline in the plaques than in healthy vessel wall (mean ratio, 2.3 ± 0.6; P = 0.014), prominently in inflamed plaques, compared with noninflamed plaque areas. Conclusion: We observed a high 11C-choline uptake in the aortic plaques of atherosclerotic mice. Our data suggest that macrophages may be responsible for the uptake of 11C-choline in the plaques.

【 授权许可】

Unknown