Journal of Nuclear Medicine | |
Isoform Selectivity of 3-125I-Iodo-α-Methyl-l-Tyrosine Membrane Transport in Human L-Type Amino Acid Transporters | |
Do Kyung Kim1  Jun Inatomi1  Nobuyoshi Ishikawa1  Naoto Shikano1  Hitoshi Endou1  Yoshikatsu Kanai1  Keiichi Kawai1  | |
关键词: membrane transport; 3-125I-iodo-α-methyl-l-tyrosine; human L-type amino acid transporter family; human 4F2hc; Xenopus laevis oocyte; | |
DOI : | |
学科分类:医学(综合) | |
来源: Society of Nuclear Medicine | |
【 摘 要 】
3-123I-Iodo-α-methyl-l-tyrosine (123I-IMT) has been developed for SPECT of amino acid transport imaging. We examined the isoform selectivity of 125I-IMT transport of the 2 human L-type amino acid transporters, hLAT1 and hLAT2, with human 4F2hc-coexpressed Xenopus laevis oocytes. Methods: An uptake study of 125I-IMT was performed using transporter-expressed X. laevis oocytes. Oocytes were injected with 17.6 ng of hLAT1 or hLAT2 complementary RNA (cRNA) and 7.4 ng of h4F2hc cRNA in a molar ratio of 1:1. Two days after injection, the uptake of 125I-IMT was measured in the Na+-free uptake solution containing 18.5 kBq of noncarrier-added 125I-IMT. After incubation for 30 min at room temperature, radioactivity of the oocytes was determined. Results: Of the 2 hLAT isoforms and h4F2hc-coexpressed X. laevis oocytes, 125I-IMT uptake via hLAT1 was 5.95-fold higher than that via hLAT2 (P < 0.005). Conclusion: 125I-IMT transport was hLAT1 selective. Investigations on the isoform selectivity of 125I-IMT transport with other transporters are anticipated.
【 授权许可】
Unknown
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