Labeling of Mixed Leukocytes with 99mTc-HMPAO Causes Severe Chromosomal Aberrations in Lymphocytes

Journal of Nuclear Medicine

Beyhan Durak¹  Ernest K.J. Pauwels¹  Marcel P.M. Stokkel¹  Zafer Gülbaş¹  İlknur Ak¹  Erkan Vardereli¹  Nurettin Basaran¹ 

关键词: ^99mTc-HMPAO;    leukocyte labeling;    radiation damage;    chromosomal aberrations;    lymphocytes;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

99mTc-d,1-hexamethylpropyleneamine oxime (HMPAO) is widely used as a labeling agent for leukocytes in the diagnosis of inflammatory or infectious foci. Cytotoxicity studies have indicated that intracellular labeling of leukocytes with 111In compounds may have severe detrimental effects on the cells. Methods: In this study, the radiotoxic effects on mixed lymphocytes after labeling with 99mTc-HMPAO was investigated using the cytokinesis-blocked micronucleus assay and chromosomal aberration assay. Results: Whereas negligible numbers of chromosome abnormalities were noted in unlabeled lymphocytes, the labeled lymphocytes showed multiple aberrations of various types, including dicentric, tricentric, and fivecentric chromosomes; centric rings; chromosome and chromatid type breaks; and acentric fragments. Conclusion: Heavily aberrant lymphocytes are seen in 99mTc-HMPAO–labeled mixed leukocytes after routine clinical procedures. It is unlikely, however, that this would cause detrimental effects, such as lymphoid malignancy, as these cells would normally be eliminated through apoptosis or phagocytosis.

【 授权许可】

Unknown