Assessment of Porcine Bone Metabolism by Dynamic [18F]Fluoride Ion PET: Correlation with Bone Histomorphometry

Journal of Nuclear Medicine

Anke Stahlschmidt¹  Georg A. Becker¹  Gerhard Maier¹  Hans-Jürgen Machulla¹  Tilman T. Zittel¹  Morand Piert¹  Roland Bares¹  Michael Jahn¹ 

[1] Departments of General Surgery and Nuclear Medicine and Section of Radiopharmacy, University of Tübingen, Tübingen, Germany Departments of General Surgery and Nuclear Medicine and Section of Radiopharmacy, University of Tübingen, Tübingen, Germany Departments of General Surgery and Nuclear Medicine and Section of Radiopharmacy, University of Tübingen, Tübingen, Germany

关键词: [¹⁸F]fluoride ion PET;    bone metabolic activity;    bone histomorphometry;    bone blood flow;    quantitative imaging;   

DOI  :  

学科分类：医学（综合）

来源: Society of Nuclear Medicine

【 摘 要 】

The aim of this study was to quantify regional bone blood flow and [18F]fluoride ion influx with [18F]fluoride ion PET and correlate the results with specific static and dynamic indices of bone metabolism in healthy pigs. Methods: During continuous ventilation (fractional concentration of oxygen in inspired gas = 0.3), dynamic PET scans 120 min in duration were obtained for 9 mini pigs after intravenous injection of 10.0 ± 1.2 MBq (mean ± SD) of [18F]fluoride ion per kilogram of body weight. Iliac crest bone biopsies were performed immediately before the PET scan to determine static and dynamic indices of bone metabolism (i.e., the mineral apposition rate) by bone histomorphometry. Kinetic rate constants describing influx (K1) and efflux (k2) of [18F]fluoride as well as chemisorption and incorporation of [18F]fluoride (k3) and reverse transport (k4) were determined for 6 vertebral bodies in each animal. Blood flow estimates (f) were derived from K1 values corrected for the permeability–surface area product using a previously derived correction algorithm. A rate constant describing the net forward transport rate of fluoride (Ki) and the fluoride volume flux (Kflux) derived from a 2-tissue-compartment model was calculated and compared with the results of Patlak graphic analysis (Kpat). Results: A significant correlation was found between mineral apposition rate and Ki (P < 0.005), Kflux (P < 0.01), Kpat, K1, and f (P < 0.05). The values of f, Ki, Kflux, and Kpat did not correlate significantly with other static or dynamic histomorphometric indices or with age, serum alkaline phosphatase, or parathyroid hormone levels. The values of f and Ki correlated linearly (y = 0.023 + 0.32x; r2 = 0.74; P < 0.001). Conclusion: PET bone studies using [18F]fluoride ion provide quantitative estimates of bone blood flow and metabolic activity that correlate with histomorphometric indices of bone formation in the normal bone tissue of the mini pig. Therefore, it seem reasonable to assume that [18F]fluoride ion PET can reduce the number of invasive bone biopsies, thus facilitating follow-up of patients with metabolic bone diseases.

【 授权许可】

Unknown